Human IL28B ELISA Kit1 Publications

カタログ番号: KE00014

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL28B ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.8 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 97% 85%-113%
Human plamsa 103% 86%-122%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 577.0 23.2 4.0
2 20 189.4 7.7 4.0
3 20 45.8 2.3 4.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 567.3 28.7 5.1
2 24 176.1 6.3 3.6
3 24 39.8 2.3 5.8

製品概要
KE00014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28B ELISA kit is to be used to detect and quantify protein levels of endogenous IL28B. The assay recognizes human IL28B. A polyclonal antibody specific for IL28B has been pre-coated onto the microwells. The IL28B protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL28B is added to detect the captured IL28B protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Cytokine Zcyto22, IFN lambda 3, IFN lambda 4, IFNL3, IFNL4, IL 28B, IL 28C, IL-28, IL28B, IL-28B, IL28C, Interferon lambda 3, Interferon lambda 4, Interleukin 28B, Interleukin 28C, ZCYTO22
背景説明

IL28B, also named as IFNL3, is a cytokine distantly related to type I interferons and the IL-10 family. IL28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).


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