Human HSP90 ELISA Kit0 Publications

カタログ番号: KE00054

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human HSP90 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Cell lysate, Urine

アッセイ方法:
Sandwich

感度:
90 pg/mL

測定範囲:
125-8000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 108% 92%-126%
Cell lysate 97% 80%-119%
Urine 105% 81%-124%
Human plasma 100% 82%-119%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 5,941 463.1 7.8
2 20 1,655.7 77.2 4.7
3 20 436.0 30.5 7.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 7,834.6 752.1 9.6
2 24 2,133.4 163.6 7.7
3 24 525.3 31.2 5.9

製品概要
KE00054 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP90 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP90. The assay recognizes human HSP90. A polyclonal antibody specific for HSP90 has been pre-coated onto the microwells. The HSP90 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSP90 is added to detect the captured HSP90 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2
背景説明

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.


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