Human HSP70 ELISA Kit0 Publications

カタログ番号: KE00059

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human HSP70 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
1.0 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 97% 80%-123%
Urine 86% 76%-98%
Human plasma 104% 92%-124%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 182.3 7.7 4.2
2 20 448.4 18.2 4.1
3 20 1,627.2 102.4 6.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 181.9 11.1 6.1
2 24 465.4 23.4 5.0
3 24 1,639.4 90.5 5.5

製品概要
KE000059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP70 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP70. The assay recognizes human HSP70. A polyclonal antibody specific for HSP70 has been pre-coated onto the microwells. The HSP70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSP70 is added to detect the captured HSP70 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B
背景説明

HSP70 is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, refolding, transport, and targeting. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Increased serum HSP70 levels are also associated with progression of multiple diseases, including atherosclerosis, heart failure, and diabetes.


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