Human F12 ELISA Kit0 Publications

カタログ番号: KE00034

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human F12 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
0.01 ng/mL

測定範囲:
0.5-32 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 94% 73%-124%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 12.18 0.77 6.40
2 20 2.90 0.19 6.70
3 20 0.55 0.03 6.00

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 12.29 1.26 10.30
2 24 2.95 0.19 6.30
3 24 0.61 0.03 5.50

製品概要
KE00034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The F12 ELISA kit is to be used to detect and quantify protein levels of endogenous F12. The assay recognizes human F12. A polyclonal antibody specific for F12 has been pre-coated onto the microwells. The F12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for F12 is added to detect the captured F12 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor
背景説明

Factor XII (FXII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. FXII plays an important role in blood coagulation, fibrinolysis, and kinin generation. When plasma comes into contact with negatively charged surfaces such as glass, kaolin, or dextran sulfate, FXII is bound to the surface and undergoes limited proteolysis by plasma kallikrein, resulting in two active enzyme forms, ɑ-FXIIa and β-FXIIa. Hereditary deficiencies in FXII are not associated with spontaneous or excessive bleeding. FXII inhibition also have protective role against arterial thrombosis and stroke, suggesting that FXII may be a target for antithrombotic therapy. This kit is used to quantify FXII in vivo.


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