Human ENO2 ELISA Kit1 Publications

カタログ番号: KE00050

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human ENO2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.11 ng/mL

測定範囲:
0.313-20 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 90% 70%-110%
Human plasma 87% 71%-118%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 2.3 0.1 6.0
2 20 7.6 0.6 7.8
3 20 16.1 0.9 5.5

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 2.3 0.1 5.7
2 24 7.4 0.4 5.6
3 24 12.9 0.8 5.8

製品概要
KE00050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ENO2 ELISA kit is to be used to detect and quantify protein levels of endogenous ENO2. The assay recognizes human ENO2. A polyclonal antibody specific for ENO2 has been pre-coated onto the microwells. The ENO2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for ENO2 is added to detect the captured ENO2 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE
背景説明

ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.


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