Human CNPY2 ELISA Kit0 Publications

カタログ番号: KE00073

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CNPY2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell lysate

アッセイ方法:
Sandwich

感度:
0.01 ng/mL

測定範囲:
0.156-10 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 88% 80%-95%
Human plasma 96% 85%-114%
Cell lysates 88 85%-91%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 0.96 0.08 8.2
2 20 1.83 0.11 6.2
3 20 6.17 0.50 8.0

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 0.61 0.05 8.7
2 24 1.74 0.17 9.5
3 24 5.20 0.47 9.1

製品概要
KE00073 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CNPY2 ELISA kit is to be used to detect and quantify protein levels of endogenous CNPY2. The assay recognizes human CNPY2. A polyclonal antibody specific for CNPY2 has been pre-coated onto the microwells. The CNPY2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CNPY2 is added to detect the captured CNPY2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
背景説明

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth (PMID: 12826659). It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 is a HIF-1α regulated, secreted angiogenic growth factor that promotes smooth muscle cells migration, proliferation and tissue revascularization (PMID: 25589425). CNPY2 enhances cell spreading in fibroblasts and migration of rat C6 glioma cells (PMID: 15905959). CNPY2 was also shown to influence the expression of low-density lipoprotein receptor and FGF21 (PMID: 22378787). This kit is used to quantify CNPY2 level in vivo.


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