IL-33 Polyclonal antibody

IL-33 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28035-1-AP

Synonyms

9230117N10Rik, Il 33, Il1f11, Il33, IL-33, interleukin 33, NF HEV



Tested Applications

Positive WB detected inmouse lung tissue, mouse colon tissue, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28035-1-AP targets IL-33 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-33 fusion protein Ag27846 相同性解析による交差性が予測される生物種
Full Name interleukin 33
Calculated molecular weight 30 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberNM_001164724
Gene symbol Il33
Gene ID (NCBI) 77125
RRIDAB_2881042
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. IL-33 activates many immune cell types involved in type-2 immunity and allergic inflammation, including ILC2s, mast cells, Th2 cells, eosinophils, basophils, dendritic cells and alternatively activated macrophages (AAM). As a cytokine, IL-33 interacts with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. IL-33 is also effective in reversing Alzheimer-like symptoms in APP/PS1 mice, by reversing the buildup and preventing the new formation of amyloid plaques. IL-33 is synthesized as a 30-34 kD full-length form and 20 kDa form mature form.

Protocols

Product Specific Protocols
WB protocol for IL-33 antibody 28035-1-APDownload protocol
Standard Protocols
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