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HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b


human, mouse, rat, hamster, zebrafish, monkey




HRP Fluorescent Dye



Cat no : HRP-66009


ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin

Tested Applications

Positive WB detected inJurkat cells, HeLa, HepG2, HEK-293, HSC-T6, NIH/3T3, 4T1 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of HRP-66009 is Beta Actin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, hamster, zebrafish, monkey
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name actin, beta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_001101
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.


Product Specific Protocols
WB protocol for HRP Beta Actin antibody HRP-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols



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