- Featured Product
- KD/KO Validated
HRP-conjugated Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, hamster, zebrafish, monkey
Applications
WB, IP
Conjugate
HRP Fluorescent Dye
CloneNo.
2D4H5
Cat no : HRP-66009
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa, HepG2, HEK-293, HSC-T6, NIH/3T3, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 20 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of HRP-66009 is Beta Actin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat, hamster, zebrafish, monkey |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fusion Protein |
| Full Name | actin, beta |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | NM_001101 |
| Gene symbol | ACTB |
| Gene ID (NCBI) | 60 |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP Beta Actin antibody HRP-66009 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol Context-Dependent Roles for SIRT2 and SIRT3 in Tumor Development Upon Calorie Restriction or High Fat Diet. | ||
FASEB J Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy. | ||
Cell Death Discov Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway. | ||
Neurosci Bull Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1. | ||
Oxid Med Cell Longev Network Pharmacology Deciphers the Action of Bioactive Polypeptide in Attenuating Inflammatory Osteolysis via the Suppression of Oxidative Stress and Restoration of Bone Remodeling Balance. | ||
Reproduction TCF3 regulates human endometrial stromal cell proliferation and migration in RPL. |
