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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A11G9

Cat no : CL488-60200

Synonyms

70 kDa zeta associated protein, FLJ17670, FLJ17679, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL488-60200

    FC experiment of Jurkat using CL488-60200

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ZAP70 (CL488-60200, Clone:1A11' target='_blank'>A11G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60200-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-60200 is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
RRIDAB_2919236
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ZAP70 antibody CL488-60200Download protocol
Standard Protocols
Click here to view our Standard Protocols