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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H9D1

Cat no : CL555-60330

Synonyms

FLJ36605, VIM, vimentin

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL555-60330

    IF Staining of HepG2 using CL555-60330

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:400.

  • IF Staining of HeLa using CL555-60330

    IF Staining of HeLa using CL555-60330

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL555-60330 targets Vimentin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
RRIDAB_2919663
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Protocols

Product Specific Protocols
IF protocol for CL555 Vimentin antibody CL555-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols