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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3A6F2

Cat no : 67219-1-Ig

Synonyms

VAMP 4, VAMP24, VAMP4

Validation Data Gallery

Applications:
  • WB analysis using 67219-1-Ig

    WB analysis using 67219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67219-1-Ig

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67219-1-Ig

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67219-1-Ig

    IF Staining of HEK-293 using 67219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:150-1:600
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

67219-1-Ig targets VAMP4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Reactivity Human, Pig, Rat, Mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VAMP4 fusion protein Ag27854 相同性解析による交差性が予測される生物種
Full Name vesicle-associated membrane protein 4
Calculated molecular weight 16 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC007019
Gene symbol VAMP4
Gene ID (NCBI) 8674
RRIDAB_2882510
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Protocols

Product Specific Protocols
WB protocol for VAMP4 antibody 67219-1-IgDownload protocol
IHC protocol for VAMP4 antibody 67219-1-IgDownload protocol
IF protocol for VAMP4 antibody 67219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

eNeuro

ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development

Authors - Yoshinobu Hara
View Article