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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCP2 Monoclonal antibody

UCP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B4B1

Cat no : 66700-1-Ig

Synonyms

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 66700-1-Ig

    WB analysis of Jurkat using 66700-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human adipose using 66700-1-Ig

    WB analysis of human adipose using 66700-1-Ig

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 66700-1-Ig

    WB analysis of human skeletal muscle using 66700-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, human adipose tissue, human skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy UCP2 Ready-To-Use IHC Kit. UCP2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66700-1-Ig targets UCP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen UCP2 fusion protein Ag19361 相同性解析による交差性が予測される生物種
Full Name uncoupling protein 2 (mitochondrial, proton carrier)
Calculated molecular weight 33 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC011737
Gene symbol UCP2
Gene ID (NCBI) 7351
RRIDAB_2882053
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.

Protocols

Product Specific Protocols
WB protocol for UCP2 antibody 66700-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols