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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated U2AF35 Monoclonal antibody

U2AF35 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B7F11

Cat no : CL488-60289

Synonyms

DKFZp313J1712, FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-60289

    IF Staining of HepG2 using CL488-60289

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-60289 (U2AF35 antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL488-60289

    FC experiment of HepG2 using CL488-60289

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human U2AF35 (CL488-60289, Clone:2B7F11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy U2AF1 Ready-To-Use IHC Kit. U2AF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60289-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60289 targets U2AF35 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen U2AF35 fusion protein Ag0399 相同性解析による交差性が予測される生物種
Full Name U2 small nuclear RNA auxiliary factor 1
Calculated molecular weight 28 kDa
GenBank accession numberBC001923
Gene symbol U2AF1
Gene ID (NCBI) 7307
RRIDAB_2883135
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 U2AF35 antibody CL488-60289Download protocol
Standard Protocols
Click here to view our Standard Protocols