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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Thymidylate synthase Polyclonal antibody

Thymidylate synthase Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-15047

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL555-15047

    IF Staining of HeLa using CL555-15047

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Thymidylate synthase antibody (CL555-15047) at dilution of 1:100.

  • FC experiment of HeLa using CL555-15047

    FC experiment of HeLa using CL555-15047

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Thymidylate synthase (CL555-15047) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15047-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL555-15047 targets Thymidylate synthase in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thymidylate synthase fusion protein Ag7027 相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
RRIDAB_2919622
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
IF protocol for CL555 Thymidylate synthase antibody CL555-15047Download protocol
Standard Protocols
Click here to view our Standard Protocols