CoraLite® Plus 488-conjugated TRAP1 Polyclonal antibody

TRAP1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10325

Synonyms

HSP 75, HSP75, HSP90L, TNFR associated protein 1, TRAP 1, TRAP1



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10325-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-10325 targets TRAP1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAP1 fusion protein Ag0400 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated protein 1
Calculated molecular weight 80 kDa
Observed molecular weight 66-70 kDa, 60 kDa
GenBank accession numberBC000406
Gene symbol TRAP1
Gene ID (NCBI) 10131
RRIDAB_2918971
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRAP1 antibody CL488-10325Download protocol
FC protocol for CL Plus 488 TRAP1 antibody CL488-10325Download protocol
Standard Protocols
Click here to view our Standard Protocols