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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

TRAIL Polyclonal antibody

TRAIL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27064-1-AP

Synonyms

Apo 2 ligand, Apo 2L, APO2L, CD253, Protein TRAIL, TL2, TNFSF10, TRAIL

Validation Data Gallery

Applications:
  • WB analysis using 27064-1-AP

    WB analysis using 27064-1-AP

    Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 27064-1-AP

    WB analysis using 27064-1-AP

    Human milk were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27064-1-AP

    IHC staining of human kidney using 27064-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 27064-1-AP

    IHC staining of human kidney using 27064-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 27064-1-AP

    IHC staining of human lymphoma using 27064-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 27064-1-AP

    IHC staining of human lymphoma using 27064-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 27064-1-AP

    IF Staining of MCF-7 using 27064-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman milk
Positive IHC detected inhuman kidney tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27064-1-AP targets TRAIL in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAIL fusion protein Ag25746 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated molecular weight 281 aa, 33 kDa
Observed molecular weight 33 kDa, 20 kDa
GenBank accession numberBC032722
Gene symbol TNFSF10
Gene ID (NCBI) 8743
RRIDAB_2880737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAIL antibody 27064-1-APDownload protocol
IHC protocol for TRAIL antibody 27064-1-APDownload protocol
IF protocol for TRAIL antibody 27064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stem Cells Transl Med

Site-Specific Integration of TRAIL in iPSC-Derived Mesenchymal Stem Cells for Targeted Cancer Therapy.

Authors - Zujia Wang
View Article
humanWB

Int J Pharm

Fusion of an EGFR-antagonistic affibody enhances the anti-tumor effect of TRAIL to EGFR positive tumors.

Authors - Dianlong Jia
View Article
humanWB

Arthritis Res Ther

IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes.

Authors - Xiaomei Yang
View Article
humanWB

Cell Biosci

Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis.

Authors - Chuan Yang
  • KD Validated
View Article
humanWB

J Nat Prod

Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission

Authors - Xiao-Jie Chen
View Article
humanWB

Phytomedicine

Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis

Authors - Li Zhang
View Article