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TPPP Monoclonal antibody

TPPP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, chicken, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E5F5

Cat no : 68048-1-Ig

Synonyms

25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1

Validation Data Gallery

Applications:
  • WB analysis using 68048-1-Ig

    WB analysis using 68048-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of SH-SY5Y using 68048-1-Ig

    WB analysis of SH-SY5Y using 68048-1-Ig

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • WB analysis of pig cerebellum using 68048-1-Ig

    WB analysis of pig cerebellum using 68048-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit cerebellum using 68048-1-Ig

    WB analysis of rabbit cerebellum using 68048-1-Ig

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat cerebellum using 68048-1-Ig

    WB analysis of rat cerebellum using 68048-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, SH-SY5Y cells, rabbit brain, rat brain, mouse brain, chicken brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68048-1-Ig targets TPPP in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, chicken, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, chicken, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPP fusion protein Ag19046 相同性解析による交差性が予測される生物種
Full Name tubulin polymerization promoting protein
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight24-29 kDa
GenBank accession numberBC131506
Gene symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Protocols

Product Specific Protocols
WB protocol for TPPP antibody 68048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols