- Featured Product
- KD/KO Validated
CoraLite®594-conjugated TPPII Monoclonal antibody
TPPII Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5H6F11
Cat no : CL594-66017
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66017-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
The immunogen of CL594-66017 is TPPII Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TPPII fusion protein Ag5296 相同性解析による交差性が予測される生物種 |
| Full Name | tripeptidyl peptidase II |
| Calculated molecular weight | 1249 aa, 138 kDa |
| Observed molecular weight | 135 kDa |
| GenBank accession number | BC039905 |
| Gene symbol | TPP2 |
| Gene ID (NCBI) | 7174 |
| RRID | AB_2883479 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TPPII antibody CL594-66017 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
