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KD/KO Validated

TOMM40 Monoclonal antibody

Name: TOMM40 Monoclonal antibody
Brand: Proteintech
SKU: 66658-1-Ig

TOMM40 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

5E2C5

Review Product

Cat no : 66658-1-Ig

Synonyms

C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40

Validation Data Gallery

Applications:

AllWBIF

WB analysis of HeLa using 66658-1-Ig
WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells.
WB analysis using 66658-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
WB analysis using 66658-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66658-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66658-1-Ig
HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 66658-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 66658-1-Ig
pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 66658-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HeLa using 66658-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66658-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Cat No. 66658-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, pig brain tissue, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW264.7 cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IF detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 6 publications below
IHC	See 1 publications below

Product Information

66658-1-Ig targets TOMM40 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity	Human, Mouse, Rat, Pig
Cited Reactivity	human, rat, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TOMM40 fusion protein Ag13065 相同性解析による交差性が予測される生物種
Full Name	translocase of outer mitochondrial membrane 40 homolog (yeast)
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC017224
Gene symbol	TOMM40
Gene ID (NCBI)	10452
RRID	AB_2882015
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TOMM40 antibody 66658-1-Ig	Download protocol
IF protocol for TOMM40 antibody 66658-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Science Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex. Authors - Qiang Wang View Article
human	WB	Mol Cell Dynamic Regulation of Mitochondrial Import by the Ubiquitin System. Authors - Lilian Phu View Article
human	WB	Elife Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress Authors - Jinyang Wang View Article
mouse	WB	Sci Signal A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation. Authors - Orna Ernst View Article
rat	WB	Apoptosis Lactate accelerates vascular calcification through NR4A1-regulated mitochondrial fission and BNIP3-related mitophagy. Authors - Yi Zhu View Article
rat	WB,IHC	Int J Mol Med 'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion. Authors - Xuan Su View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TOMM40 Monoclonal antibody

TOMM40 Monoclonal Antibody for IF, WB, ELISA

Validation Data Gallery

WB analysis of HeLa using 66658-1-Ig

WB analysis using 66658-1-Ig

WB analysis using 66658-1-Ig

WB analysis of HEK-293 using 66658-1-Ig

WB analysis of HepG2 using 66658-1-Ig

WB analysis of human brain using 66658-1-Ig

WB analysis of pig brain using 66658-1-Ig

IF Staining of HepG2 using 66658-1-Ig

IF Staining of HeLa using 66658-1-Ig

IF Staining of HepG2 using 66658-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation.

Lactate accelerates vascular calcification through NR4A1-regulated mitochondrial fission and BNIP3-related mitophagy.

'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion.