- Featured Product
- KD/KO Validated
TMEM106B Monoclonal antibody
TMEM106B Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5D1F8
Cat no : 60333-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
60333-1-Ig targets TMEM106B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TMEM106B fusion protein Ag21448 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 106B |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | ~40 kDa |
| GenBank accession number | BC033901 |
| Gene symbol | TMEM106B |
| Gene ID (NCBI) | 54664 |
| RRID | AB_2881442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
| IP protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes | ||
Pathol Res Pract TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinoma | ||
Acta Neuropathol Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging | ||
Front Cell Neurosci Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation
|
