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  • Featured Product
  • KD/KO Validated

TIMM44 Monoclonal antibody

TIMM44 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4G7D4

Cat no : 66149-1-Ig

Synonyms

MIMT44, TIM44, TIMM44

Validation Data Gallery

Applications:
  • WB analysis using 66149-1-Ig

    WB analysis using 66149-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66149-1-Ig (TIMM44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 66149-1-Ig

    WB analysis of HeLa using 66149-1-Ig

    WB result of TIMM44 antibody (66149-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TIMM44 transfected HeLa cells.

  • IHC staining of human liver using 66149-1-Ig

    IHC staining of human liver using 66149-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66149-1-Ig

    IHC staining of human liver using 66149-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66149-1-Ig (TIMM44 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66149-1-Ig

    IF Staining of HepG2 using 66149-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIMM44 antibody (66149-1-Ig, Clone: 4G7D4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rabbit heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66149-1-Ig targets TIMM44 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen TIMM44 fusion protein Ag21389 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 44 homolog (yeast)
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC033628
Gene symbol TIMM44
Gene ID (NCBI) 10469
RRIDAB_2881545
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TIMM44 antibody 66149-1-IgDownload protocol
IHC protocol for TIMM44 antibody 66149-1-IgDownload protocol
IF protocol for TIMM44 antibody 66149-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Lana (Verified Customer) (04-20-2019)

SDS-PAGE:15 ug/ul RIPA lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 800CW Goat anti-Mouse1 h incubation at room temperatureLines on WB:1. BioRad Precision Plus Protein standards2. Lysate of mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: ST-Hdh mouse striatal cell line
TIMM44 Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:66149-1-Ig)