TBR1 Monoclonal antibody
TBR1 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse, pig and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5C3A5
Cat no : 66564-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, rat cerebellum tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy TBR1 Ready-To-Use IHC Kit. TBR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
66564-1-Ig targets TBR1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.
| Tested Reactivity | Human, rat, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TBR1 fusion protein Ag15262 相同性解析による交差性が予測される生物種 |
| Full Name | T-box, brain, 1 |
| Calculated molecular weight | 682 aa, 74 kDa |
| Observed molecular weight | 74 kDa |
| GenBank accession number | BC104844 |
| Gene symbol | TBR1 |
| Gene ID (NCBI) | 10716 |
| RRID | AB_2881925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| IHC protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| IF protocol for TBR1 antibody 66564-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis. | ||
EMBO J Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway. | ||
Biochem Biophys Res Commun Neuron-specific loss of Ppp6c induces neonatal death and decreases the number of cortical neurons and interneurons | ||
