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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Stanniocalcin 2 Monoclonal antibody

Stanniocalcin 2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H1E7

Cat no : CL594-60063

Synonyms

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60063

    IF Staining of HeLa using CL594-60063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60063 (Stanniocalcin 2 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-60063

    FC experiment of HeLa using CL594-60063

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Stanniocalcin 2 (CL594-60063, Clone:4H1E7) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STC2 Ready-To-Use IHC Kit. STC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60063-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-60063 targets Stanniocalcin 2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stanniocalcin 2 fusion protein Ag0359 相同性解析による交差性が予測される生物種
Full Name stanniocalcin 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC000658
Gene symbol STC2
Gene ID (NCBI) 8614
RRIDAB_2883414
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Protocols

Product Specific Protocols
IF protocol for CL594 Stanniocalcin 2 antibody CL594-60063Download protocol
Standard Protocols
Click here to view our Standard Protocols