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  • KD/KO Validated

SRRM1 Polyclonal antibody

SRRM1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12822-1-AP

Synonyms

160 KD, POP101, SRM160, SRRM1



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, Jurkat cells, UV treated HEK-293
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SRRM1 Ready-To-Use IHC Kit. SRRM1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

12822-1-AP targets SRRM1 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRRM1 fusion protein Ag3513 相同性解析による交差性が予測される生物種
Full Name serine/arginine repetitive matrix 1
Calculated molecular weight 904 aa, 102 kDa
Observed molecular weight 120-160 kDa
GenBank accession numberBC036187
Gene symbol SRRM1
Gene ID (NCBI) 10250
RRIDAB_2286613
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.

Protocols

Product Specific Protocols
WB protocol for SRRM1 antibody 12822-1-APDownload protocol
IP protocol for SRRM1 antibody 12822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Host Microbe

Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry.

Authors - Wojcechowskyj Jason A JA
  • KD Validated
humanWB

Biochim Biophys Acta Mol Cell Res

Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy.

Authors - Taiki Baba
humanCoIP

Tissue Cell

SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway

Authors - Xiaoyan Song