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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPON2 Monoclonal antibody

SPON2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H4B11

Cat no : 66859-1-Ig

Synonyms

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

Validation Data Gallery

Applications:
  • WB analysis of LNCaP using 66859-1-Ig

    WB analysis of LNCaP using 66859-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66859-1-Ig

    IHC staining of human prostate cancer using 66859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66859-1-Ig

    IHC staining of human prostate cancer using 66859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66859-1-Ig targets SPON2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SPON2 fusion protein Ag14337 相同性解析による交差性が予測される生物種
Full Name spondin 2, extracellular matrix protein
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC002707
Gene symbol SPON2
Gene ID (NCBI) 10417
RRIDAB_2882198
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

Protocols

Product Specific Protocols
WB protocol for SPON2 antibody 66859-1-IgDownload protocol
IHC protocol for SPON2 antibody 66859-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Bone

SPON2 promotes the bone metastasis of lung adenocarcinoma via activation of the NF-κB signaling pathway

Authors - Ming Wu
  • KD Validated
View Article