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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated SPATA22 Polyclonal antibody

SPATA22 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16989

Synonyms

NYD SP20, SPATA22, spermatogenesis associated 22

Validation Data Gallery

Applications:
  • IF Staining of mouse testis using CL594-16989

    IF Staining of mouse testis using CL594-16989

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CL594-16989 (SPATA22 antibody) at dilution of 1:50.

Tested Applications

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 16989-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-16989 targets SPATA22 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATA22 fusion protein Ag10591 相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 22
Calculated molecular weight 363 aa, 41 kDa
GenBank accession numberBC029483
Gene symbol SPATA22
Gene ID (NCBI) 84690
RRIDAB_2883406
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks. This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL594 SPATA22 antibody CL594-16989Download protocol
Standard Protocols
Click here to view our Standard Protocols