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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRPN Monoclonal antibody

SNRPN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

1H2F4

Cat no : 67280-1-Ig

Synonyms

DKFZp686C0927, DKFZp686M12165, DKFZp761I1912, DKFZp762N022, FLJ33569, FLJ36996, FLJ39265, HCERN3, PWCR, RT LI, SM D, Sm N, Sm protein D, Sm protein N, SMN, SNRNP N, SNRPN, SNURF SNRPN

Validation Data Gallery

Applications:
  • WB analysis using 67280-1-Ig

    WB analysis using 67280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67280-1-Ig targets SNRPN in WB, IHC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNRPN fusion protein Ag14030 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide N
Calculated molecular weight 240 aa, 25 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC000611
Gene symbol SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

Protocols

Product Specific Protocols
WB protocol for SNRPN antibody 67280-1-IgDownload protocol
IHC protocol for SNRPN antibody 67280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols