• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMARCD1 Monoclonal antibody

SMARCD1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2F2G6

Cat no : 66116-1-Ig

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 66116-1-Ig

    WB analysis of Jurkat using 66116-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 66116-1-Ig

    WB analysis of human brain using 66116-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66116-1-Ig

    WB analysis of HepG2 using 66116-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, human brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66116-1-Ig is SMARCD1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMARCD1 fusion protein Ag19100 相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC009368
Gene symbol SMARCD1
Gene ID (NCBI) 6602
RRIDAB_2881515
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for SMARCD1 antibody 66116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols