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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SLC25A36 Monoclonal antibody

SLC25A36 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5E4

Cat no : 67896-1-Ig

Synonyms

DKFZp564C053, FLJ10618, SLC25A36

Validation Data Gallery

Applications:
  • WB analysis using 67896-1-Ig

    WB analysis using 67896-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67896-1-Ig (SLC25A36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human breast cancer using 67896-1-Ig

    IHC staining of human breast cancer using 67896-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67896-1-Ig (SLC25A36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67896-1-Ig

    IHC staining of human breast cancer using 67896-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67896-1-Ig (SLC25A36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67896-1-Ig targets SLC25A36 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC25A36 fusion protein Ag23894 相同性解析による交差性が予測される生物種
Full Name solute carrier family 25, member 36
GenBank accession numberBC014064
Gene symbol SLC25A36
Gene ID (NCBI) 55186
RRIDAB_2918652
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SLC25A36 antibody 67896-1-IgDownload protocol
IHC protocol for SLC25A36 antibody 67896-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Hyperinsulinism / hyperammonemia syndrome caused by biallelic SLC25A36 mutation

Authors - Amit Safran
View Article