SLC22A7 Monoclonal antibody
SLC22A7 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3D3C3
Cat no : 67479-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SMMC-7721 cells |
| Positive IHC detected in | human liver tissue, human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Product Information
67479-1-Ig targets SLC22A7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SLC22A7 fusion protein Ag25234 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 22 (organic anion transporter), member 7 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC033805 |
| Gene symbol | SLC22A7 |
| Gene ID (NCBI) | 10864 |
| RRID | AB_2882706 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC22A7 antibody 67479-1-Ig | Download protocol |
| IHC protocol for SLC22A7 antibody 67479-1-Ig | Download protocol |
| IF protocol for SLC22A7 antibody 67479-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
