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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F3B2

Cat no : CL488-60075

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, Pnutl3, SEPT2, Septin 2, septin2

Validation Data Gallery

Applications:
  • FC experiment of K-562 using CL488-60075

    FC experiment of K-562 using CL488-60075

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Septin 2 (CL488-60075, Clone:2F3B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60075-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60075 targets Septin 2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 2 fusion protein Ag1946 相同性解析による交差性が予測される生物種
Full Name septin 2
Calculated molecular weight 41 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC014455
Gene symbol SEPT2
Gene ID (NCBI) 4735
RRIDAB_2883096
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Septin 2 antibody CL488-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols