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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-22015

Synonyms

DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-22015

    IF Staining of HepG2 using CL594-22015

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated RRBP1 antibody (CL594-22015) at dilution of 1:200.

  • FC experiment of HeLa using CL594-22015

    FC experiment of HeLa using CL594-22015

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RRBP1 (CL594-22015) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22015-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-22015 is RRBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRBP1 fusion protein Ag16648 相同性解析による交差性が予測される生物種
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Calculated molecular weight 1410 aa, 152 kDa
Observed molecular weight 180 kDa, 109 kDa
GenBank accession numberBC128577
Gene symbol RRBP1
Gene ID (NCBI) 6238
RRIDAB_2919866
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

Protocols

Product Specific Protocols
IF protocol for CL594 RRBP1 antibody CL594-22015Download protocol
Standard Protocols
Click here to view our Standard Protocols