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KD/KO Validated

ROCK2(middle) Polyclonal antibody

Name: ROCK2(middle) Polyclonal antibody
Brand: Proteintech
SKU: 21645-1-AP

ROCK2(middle) Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21645-1-AP

Synonyms

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
WB analysis of HT-1080 using 21645-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
WB analysis of mouse brain using 21645-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21645-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21645-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21645-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21645-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21645-1-AP
IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 21645-1-AP
Immunofluorescent analysis of Hela cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 21645-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 15 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

The immunogen of 21645-1-AP is ROCK2(middle) Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ROCK2(middle) fusion protein Ag16330 相同性解析による交差性が予測される生物種
Full Name	Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight	77 kDa, 161 kDa
Observed molecular weight	161 kDa
GenBank accession number	BC111801
Gene symbol	ROCK2
Gene ID (NCBI)	9475
RRID	AB_10858624
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

Protocols

Product Specific Protocols
WB protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IHC protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IF protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IP protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Oncotarget Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis. Authors - Rachel Hiu Ha Ching View Article
human	WB	Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. Authors - Kathryn N Becker View Article
rat	WB	Front Pharmacol Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus. Authors - Penghui Yuan View Article
mouse	WB	Mol Pharmacol Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression. Authors - Yanhua Wang View Article
rat	WB	J Sex Med FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis. Authors - Kai Cui View Article
rat	WB,IHC	Andrology JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis. Authors - K Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Validation Data Gallery

WB analysis of HeLa using 21645-1-AP

WB analysis of HT-1080 using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of mouse brain using 21645-1-AP

WB analysis of mouse lung using 21645-1-AP

WB analysis of MCF-7 using 21645-1-AP

WB analysis of mouse liver using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of L02 using 21645-1-AP

IP experiment of HeLa using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IF Staining of HepG2 using 21645-1-AP

IF Staining of HeLa using 21645-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.

Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression.

FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.