RNF14 Polyclonal antibody

RNF14 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26368-1-AP

Synonyms

ARA54, HFB30, HRIHFB2038, ring finger protein 14, RNF14, TRIAD2, Triad2 protein



Tested Applications

Positive WB detected inHEK-293T cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26368-1-AP is RNF14 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF14 fusion protein Ag23734 相同性解析による交差性が予測される生物種
Full Name ring finger protein 14
Observed molecular weight 47-54 kDa
GenBank accession numberBC126185
Gene symbol RNF14
Gene ID (NCBI) 9604
RRIDAB_2880494
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF14 antibody 26368-1-APDownload protocol
IHC protocol for RNF14 antibody 26368-1-APDownload protocol
IF protocol for RNF14 antibody 26368-1-APDownload protocol
IP protocol for RNF14 antibody 26368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols