RNase T2 Polyclonal antibody
RNase T2 Polyclonal Antibody for IF, IHC, IP,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13753-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | transfected cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IHC | See 1 publications below |
Product Information
13753-1-AP targets RNase T2 in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNase T2 fusion protein Ag4695 相同性解析による交差性が予測される生物種 |
| Full Name | ribonuclease T2 |
| Calculated molecular weight | 256 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC039713 |
| Gene symbol | RNASET2 |
| Gene ID (NCBI) | 8635 |
| RRID | AB_2285339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RNase T2 antibody 13753-1-AP | Download protocol |
| IP protocol for RNase T2 antibody 13753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FEBS J Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import. | ||
Cell Transplant Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer. | ||
Cell Signal Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis | ||
