RIC8A Monoclonal antibody

RIC8A Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66625-1-Ig

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66625-1-Ig targets RIC8A in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RIC8A fusion protein Ag1593 相同性解析による交差性が予測される生物種
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated molecular weight 60 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011821
Gene symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2881985
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Protocols

Product Specific Protocols
WB protocol for RIC8A antibody 66625-1-IgDownload protocol
IHC protocol for RIC8A antibody 66625-1-IgDownload protocol
IF protocol for RIC8A antibody 66625-1-IgDownload protocol
IP protocol for RIC8A antibody 66625-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols