RDM1 Monoclonal antibody

RDM1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C10B4

Cat no : 66941-1-Ig

Synonyms

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1



Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HSC-T6 cells, RAW 264.7 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66941-1-Ig targets RDM1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RDM1 fusion protein Ag28686 相同性解析による交差性が予測される生物種
Full Name RAD52 motif 1
Calculated molecular weight 284 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC038301
Gene symbol RDM1
Gene ID (NCBI) 201299
RRIDAB_2882265
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

Protocols

Product Specific Protocols
WB protocol for RDM1 antibody 66941-1-IgDownload protocol
IHC protocol for RDM1 antibody 66941-1-IgDownload protocol
IF protocol for RDM1 antibody 66941-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols