• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11614

Synonyms

DKFZp547K0918, hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-11614

    IF Staining of HeLa using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-11614

    IF Staining of HepG2 using CL594-11614

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated RBM4 antibody (CL594-11614) at dilution of 1:100.

  • FC experiment of HeLa using CL594-11614

    FC experiment of HeLa using CL594-11614

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBM4 (CL594-11614) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11614-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-11614 targets RBM4 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 4
Calculated molecular weight 40 kDa
GenBank accession numberBC021120
Gene symbol RBM4
Gene ID (NCBI) 5936
RRIDAB_2919770
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Protocols

Product Specific Protocols
IF protocol for CL594 RBM4 antibody CL594-11614Download protocol
Standard Protocols
Click here to view our Standard Protocols