• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3D5E11

Cat no : 66015-2-Ig

Synonyms

BOG, RBBP9

Validation Data Gallery

Applications:
  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 66015-2-Ig

    WB analysis of A549 using 66015-2-Ig

    WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of A549 using 66015-2-Ig

    IF Staining of A549 using 66015-2-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A549 cells using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66015-2-Ig

    FC experiment of HeLa using 66015-2-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inpig brain tissue, HeLa cells, A375 cells, A549 cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66015-2-Ig targets RBBP9 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RBBP9 fusion protein Ag17864 相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 9
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC015938
Gene symbol RBBP9
Gene ID (NCBI) 10741
RRIDAB_11044197
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

Protocols

Product Specific Protocols
WB protocol for RBBP9 antibody 66015-2-IgDownload protocol
IHC protocol for RBBP9 antibody 66015-2-IgDownload protocol
IF protocol for RBBP9 antibody 66015-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols