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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB18 Monoclonal antibody

RAB18 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4B3

Cat no : 67659-1-Ig

Synonyms

RAB18, RAB18LI1, Ras related protein Rab 18

Validation Data Gallery

Applications:
  • WB analysis using 67659-1-Ig

    WB analysis using 67659-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67659-1-Ig (RAB18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67659-1-Ig

    IHC staining of mouse brain using 67659-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67659-1-Ig

    IHC staining of mouse brain using 67659-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67659-1-Ig (RAB18 antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:175-1:700
Sample-dependent, check data in validation data gallery

Product Information

67659-1-Ig targets RAB18 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB18 fusion protein Ag16669 相同性解析による交差性が予測される生物種
Full Name RAB18, member RAS oncogene family
Calculated molecular weight 206 aa, 23 kDa
GenBank accession numberBC015014
Gene symbol RAB18
Gene ID (NCBI) 22931
RRIDAB_2882856
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB18 antibody 67659-1-IgDownload protocol
IHC protocol for RAB18 antibody 67659-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols