Phospho-P53 (Ser15) Monoclonal antibody

Phospho-P53 (Ser15) Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6G1

Cat no : 67826-1-Ig

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser15), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Tested Applications

Positive WB detected inCalyculin A treated HT-29 cells, HT-29 cells, HEK-293T cells, HEK-293T cells, Etoposide treated HT-29 cells
Positive IF detected inetoposide treated HT-29 cells
Positive FC detected inUV treated HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67826-1-Ig targets Phospho-P53 (Ser15) in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
RRIDAB_2918588
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocols

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 67826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

Hepatitis B doubly spliced protein (HBDSP) promotes hepatocellular carcinoma cell apoptosis via ETS1/GATA2/YY1-mediated p53 transcription

Authors - Xiazhen Xu
humanWB

Front Pharmacol

A New Chalcone Derivative C49 Reverses Doxorubicin Resistance in MCF-7/DOX Cells by Inhibiting P-Glycoprotein Expression.

Authors - Ting Wang
humanWB

Toxicol Lett

The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

Authors - Xiaoran Guan
ratWB

World Neurosurg

N-acetylserotonin protects rat nucleus pulposus cells against oxidative stress injury by activating the PI3K/AKT signaling pathway

Authors - Wang Yidian
humanWB

Front Pharmacol

Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC

Authors - Kaixuan Wang
humanWB

Discov Oncol

Anticancer efficacy of hirsuteine against colorectal cancer by opposite modulation of wild-type and mutant p53

Authors - Yan Zhang