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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

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PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7F7

Cat no : 66810-1-Ig

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

Validation Data Gallery

Applications:
  • WB analysis using 66810-1-Ig

    WB analysis using 66810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66810-1-Ig

    WB analysis of HEK-293 using 66810-1-Ig

    WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66810-1-Ig targets PRDX3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitypig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PRDX3 fusion protein Ag1062 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 3
Calculated molecular weight 27 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC007062
Gene symbol PRDX3
Gene ID (NCBI) 10935
RRIDAB_2882153
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 66810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Free Radic Biol Med

Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling.

Authors - Yanan Chen
View Article