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  • KD/KO Validated

PRMT1 Polyclonal antibody

PRMT1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11279-1-AP

Synonyms

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1



Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11279-1-AP targets PRMT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT1 fusion protein Ag1790 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 1
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC019268
Gene symbol PRMT1
Gene ID (NCBI) 3276
RRIDAB_2171319
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 11279-1-APDownload protocol
IHC protocol for PRMT1 antibody 11279-1-APDownload protocol
IF protocol for PRMT1 antibody 11279-1-APDownload protocol
IP protocol for PRMT1 antibody 11279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Cell Metab

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners

Authors - Qing Li
humanIHC

Oncogene

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

Authors - Xin-Ke Yin
human,mouseWB,IHC

NPJ Precis Oncol

The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer

Authors - Xiangwei Zeng
mouseWB

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
mouseWB,IF

Front Mol Neurosci

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein

Authors - Cheng Wu
  • KD Validated
humanWB

Toxicol Appl Pharmacol

Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5

Authors - Yizhen Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs