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- KD/KO Validated
PRKACA Monoclonal antibody
PRKACA Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
CloneNo.
4G10D7
Cat no : 67491-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
67491-1-Ig targets PRKACA in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | PRKACA fusion protein Ag26400 相同性解析による交差性が予測される生物種 |
Full Name | protein kinase, cAMP-dependent, catalytic, alpha |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 38-43 kDa |
GenBank accession number | BC039846 |
Gene symbol | PRKACA |
Gene ID (NCBI) | 5566 |
RRID | AB_2882716 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Protocols
Product Specific Protocols | |
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WB protocol for PRKACA antibody 67491-1-Ig | Download protocol |
IHC protocol for PRKACA antibody 67491-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |