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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2CA Monoclonal antibody

PPP2CA Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A6H5

Cat no : 67809-1-Ig

Synonyms

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

Validation Data Gallery

Applications:
  • WB analysis using 67809-1-Ig

    WB analysis using 67809-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67809-1-Ig (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67809-1-Ig

    IHC staining of human liver using 67809-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67809-1-Ig

    IHC staining of human liver using 67809-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67809-1-Ig targets PPP2CA in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight33 kDa
GenBank accession numberBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2918572
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
WB protocol for PPP2CA antibody 67809-1-IgDownload protocol
IHC protocol for PPP2CA antibody 67809-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma

Authors - Yue Li
View Article