Plakophilin 3 Polyclonal antibody

Plakophilin 3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18338-1-AP

Synonyms

PKP3, plakophilin 3



Tested Applications

Positive WB detected inK-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18338-1-AP targets Plakophilin 3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plakophilin 3 fusion protein Ag13062 相同性解析による交差性が予測される生物種
Full Name plakophilin 3
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC000081
Gene symbol PKP3
Gene ID (NCBI) 11187
RRIDAB_2164281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IHC protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IP protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

J Ovarian Res

Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis

Authors - Yuan Li
humanIHC

Biomedicines

Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis

Authors - Yan Du
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang