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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated PKC Gamma Polyclonal antibody

PKC Gamma Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14364

Synonyms

PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14

Validation Data Gallery

Applications:
  • WB analysis using CL647-14364

    WB analysis using CL647-14364

    Various lysates were subjected to SDS PAGE followed by western blot with CL647-14364 (PKC Gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
For other applications, we recommend the unconjugated version of this antibody, 14364-1-AP

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

CL647-14364 targets PKC Gamma in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Gamma fusion protein Ag5681 相同性解析による交差性が予測される生物種
Full Name protein kinase C, gamma
Calculated molecular weight 78 kDa
Observed molecular weight 76-80 kDa
GenBank accession numberBC047876
Gene symbol PRKCG
Gene ID (NCBI) 5582
RRIDAB_2920225
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

Protocols

Product Specific Protocols
WB protocol for CL Plus 647 PKC Gamma antibody CL647-14364Download protocol
Standard Protocols
Click here to view our Standard Protocols