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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PGC1a Monoclonal antibody

PGC1a Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C1B2

Cat no : CL594-66369

Synonyms

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-66369

    IF Staining of HeLa using CL594-66369

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 C1' target='_blank'>PGC1a antibody (CL594-66369, Clone: 1C1B2 ) at dilution of 1:100.

  • FC experiment of HeLa using CL594-66369

    FC experiment of HeLa using CL594-66369

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human C1' target='_blank'>PGC1a (CL594-66369, Clone:1C1B2) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66369-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66369 targets PGC1a in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Calculated molecular weight 91 kDa
GenBank accession numberNM_013261
Gene symbol PPARGC1A
Gene ID (NCBI) 10891
RRIDAB_2919982
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists a isoforms in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Protocols

Product Specific Protocols
IF protocol for CL594 PGC1a antibody CL594-66369Download protocol
FC protocol for CL594 PGC1a antibody CL594-66369Download protocol
Standard Protocols
Click here to view our Standard Protocols