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CoraLite®594-conjugated PDH E1 Alpha Monoclonal antibody

PDH E1 Alpha Monoclonal Antibody for FC (Intra), WB

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B3C10

Cat no : CL594-66119

Synonyms

PDH E1 Alpha, PDHA, PDHA1, PDHCE1A, PDHE1 A type I, PHE1A



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66119-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66119 targets PDH E1 Alpha in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PDH E1 Alpha fusion protein Ag12556 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002406
Gene symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2919949
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Protocols

Product Specific Protocols
WB protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
FC protocol for CL594 PDH E1 Alpha antibody CL594-66119Download protocol
Standard Protocols
Click here to view our Standard Protocols