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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PBK Polyclonal antibody

PBK Polyclonal Antibody for FC (intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16110

Synonyms

Cancer/testis antigen 84, CT84, FLJ14385, MAPKK like protein kinase, Nori 3, PBK, PDZ binding kinase, SPK, TOPK

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-16110

    IF Staining of HepG2 using CL488-16110

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PBK antibody (CL488-16110) at dilution of 1:400.

  • FC experiment of HepG2 using CL488-16110

    FC experiment of HepG2 using CL488-16110

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PBK (CL488-16110) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16110-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-16110 targets PBK in IF, FC (intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBK fusion protein Ag9033 相同性解析による交差性が予測される生物種
Full Name PDZ binding kinase
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC015191
Gene symbol PBK
Gene ID (NCBI) 55872
RRIDAB_2919131
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PBK antibody CL488-16110Download protocol
FC protocol for CL Plus 488 PBK antibody CL488-16110Download protocol
Standard Protocols
Click here to view our Standard Protocols